Since the establishment of DNA profiling as a technique for genetic identification, many changes and adaptations have been implemented to increase the efficiency and success of DNA analysis techniques, from blood to trace to the recent introduction of new more sensitive DNA analysis kits. This has also contributed to the significant increases in the DNA casework backlogs in forensic laboratories globally, as more and more samples are required to be analysed. One process adopted by some laboratories is the reduction in samples analysed per volume crime case to one sample. This poster outlines a study examining the validity of this approach.